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There is no better platform for creating change than the direct selling industry. We believe that when thousands of people around the world are performing small, random acts of kindness, the positive impact we can create on the world each day is profound.phytoncide air purifier Volume 46, Issue 12, December 2011, Pages 2291–2300enso aroma diffuser air purifier humidifier Protease inhibitor from Moringa oleifera leaves: Isolation, purification, and characterizationa b c Received 10 December 2010, Revised 3 August 2011, Accepted 13 September 2011, Available online 17 September 2011Protease inhibitors have great demand in medicine and biotechnology. oreck air purifier air12guWe report here the purification and characterization of a protease inhibitor isolated from mature leaf extract of Moringa oleifera that showed maximum inhibitor activity.

The protease inhibitor was purified to 41.4-fold by Sephadex G75 and its molecular mass was calculated as 23,600 Da. Inhibitory activity was confirmed by dot-blot and reverse zymogram analyses. Glycine, glutamic acid, alanine, proline and aspartic acid were found as the major amino acids of the inhibitor protein. Maximal activity was recorded at pH 7 and at 40 °C. The inhibitor was stable over pH 5–10; and at 50 °C for 2 h. Thermostability was promoted by CaCl2, BSA and sucrose. Addition of Zn2+ and Mg2+, SDS, dithiothreitol and β-mercaptoethanol enhanced inhibitory activity, while DMSO and H2O2 affected inhibitory activity. Modification of amino acids at the catalytic site by PMSF and DEPC led to an enhancement in the inhibitory activity. Stoichiometry of trypsin–protease inhibitor interaction was 1:1.5 and 0.6 nM of inhibitor effected 50% inhibition. The low Ki value (1.5 nM) obtained indicated scope for utilization of M. oliefera protease inhibitor against serine proteases.

Highlights► We report here the isolation, purification, and characterization of a protease inhibitor isolated from mature leaf extract of Moringa oleifera. ► Maximal inhibitor activity was recorded at pH 7 and at 40 °C. ► The inhibitor was stable over pH 5–10; and at 50 °C for 2 h. ► The low Ki value (1.5 nM) obtained indicated scope for utilization of M. oliefera protease inhibitor against serine proteases.Copyright © 2011 Elsevier Ltd. Area code and zip code in Hainan Updated: 01 Jan 2011 Share this news?... Previous News: Chinese zodiac - 2011, year of the rabbitNext News: Coconut carvings - An historical art in Hainan province You are now in Useful Info Other articles you maybe interested Sanya's friend circle -- international friendship cities Major sea ports in Hainan Travel Popular locations for wedding photoshoot in Sanya Travel WOS Monthly Focus for Feb: Sanya golf industry WOS Monthly Focus for Jan: Sanya medical & health services

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